Day 1 :
Research Institute of Horticulture, Poland
Keynote: The influence of bio-fertilizers enriched with beneficial bacteria and filamentous fungi on the growth and rhizosphere microbiology of Marmolada strawberry plants in container cultivation
Time : 09:00 AM - 10:00 AM
Organization: Department of Soil and Orchard Management, Rhizosphere Laboratory (IO). Experience in research projects and R&D projects: CEAF, CRAFT, EraNet RURAGRI, HortiEnergia, Huntsman, EFSA carried out in collaboration with academic and industrial partners in Poland and abroad. Coordinator and executor of tasks of the research and development project EkoTechProdukt co-financed by the European Commission from the European Regional Development Fund, (2009-2015). Research interests & Achievements in implementation of R&D results and innovative activities: studies in the area of rhizosphere and nutrient management strategies in fruit crops, development of microbial inocula for horticultural production, development of bioproducts for fruit crops. Achievements in the field of industrial property rights: patents, patent applications: development of 6 new microbial consortia and 5 new bioproducts for use in organic fruit growing. Other achievements: member of the WGs of Cost Action 836 (2002-2004), member of the MCs of 3 COST Actions: 631 (2002-2005), E38 (2005-2008), TD 1107 (20012-2016), expert representing Poland in the Programme Committee of the European Commission in FP6 (2002-2006) and in the Programme Committee of the 7th EU Framework Programme (2007-2012), expert of EFSA – European Food Safety Authority (2007-2012), member of the Management Committee and Working Group of COST Action ES 1406 ‘Soil fauna - Key to Soil Organic Matter Dynamics and Modelling (KEYSOM)’ (2015-2018), expert evaluating research projects/reports of the 6th and 7th EU Framework Programmes (2002-2013) and Horizon 2020 (2014-2020)
The experiment in stoneware pots was established in the spring of 2018 in four replications in the experimental field of the Warsaw University of Life Sciences in Skierniewice. The objects studied were strawberry plants of the cultivar Marmolada. The experiment was conducted in stoneware pots with a diameter of 40 cm filled with about 270 liters of arable soil with a pH of 6.2, to each of which three seedlings of the Frigo A+ type (15-18 mm) strawberry plants were planted out at the beginning of May. Each combination consisted of six replications (stoneware pots). The experiment was established in a random block design with a total of 13 experimental combinations, including beneficial microorganisms and filamentous fungi, and a no-treatment (zero) control. In addition, the Marmolada strawberry plants were divided into two experimental groups: In one of them the plants were grown in optimal irrigation conditions (100% of the water dose) and in the other, from mid-June, under drought stress (50% of the water dose). In both groups, the same fertilization was applied. The experiment included the following experimental combinations: (1) Control-plants not fertilized (2) Standard NPK fertilization (3) Control with the addition of filamentous fungi (Aspergillus niger and Paecilomyces lilacinus) (4) Control-with the addition of beneficial Bacillus bacteria (Bacillus sp., Bacillus amyloliquefaciens and Paenibacillus polymyxa) (5) Standard NPK + filamentous fungi (6) Standard NPK + beneficial bacteria (7) 100% Polifoska 6 + beneficial bacteria (8) 100% Urea + filamentous fungi (9) 100% Polifoska 6 enriched with beneficial bacteria (10) 100% Fos Dar 40 enriched with beneficial bacteria (11) 60% Urea enriched with filamentous fungi (12) 60% Polifoska 6 enriched with beneficial bacteria (13) Fos Dar 40 in a 60% dose enriched with three strains of beneficial bacteria. In 2018, yielding was not assessed because all inflorescences were removed. The amounts of minerals (macro and micronutrients) were determined in strawberry leaves. In the autumn, runners were collected for assessment, where the number, fresh weight, length, the number of runner plants and their fresh weight were determined. The results of the first year of the study showed that filamentous fungi and beneficial bacteria applied together with mineral fertilizers tested on strawberry plants (Urea, Polifoska 6, Fos Dar 40) increased their biological activity. This was particularly evident in the development of the aboveground parts of strawberry plants (runners and runner plants, and the concentration of some minerals, macro- and microelements, in the leaves). The amount of water supplied to the strawberry plants fertilized with various mineral fertilizers enriched with beneficial bacteria and fungi had a greater impact on the growth characteristics of the aboveground parts of the plants when using the full dose of water (100%) than the dose of water reduced by half (50%). The microbiological analysis of the soil showed that the microbially enriched mineral fertilizers Polifoska 6 and Fos Dar 40 (with the addition of beneficial Bacillus bacteria: Bacillus sp., Bacillus amyloliquefaciens and Paenibacillus polymyxa) and Urea at 100% applied together with filamentous fungi (Paecilomyces lilacinus, Aspergillus niger) had a favorable effect on increasing the numbers of beneficial microorganisms in the rhizosphere soil of Marmolada strawberry plants
Hiroshima University, Japan
Keynote: First report of Klebsiella pneumoniae co-producing NDM-1 and VIM-1 carbapenemases from a meat sample in Japan
Time : 10:00 AM - 10:45 AM
Tadashi Shimamoto has completed his PhD from Okayama University, Japan and Postdoctoral studies from University of Medicine and Dentistry of New Jersey, Robert Wood Johnson Medical School, USA. He is a Professor of Laboratory of Food Microbiology and Hygiene, Graduate School of Integrated Sciences for Life, Hiroshima University, Japan. He has published more than 80 papers in reputed journals.
Carbapenems are a class of highly potent antibiotics that are commonly used as last-resort antibiotics for treatment of severe infections caused by multidrug-resistant bacteria. Therefore, carbapenem antibiotics are not licensed for food-producing animals in many countries, only for human use. This study was designed to elucidate the incidence and molecular characterization of foodborne carbapenemases producing bacteria in Japan. A total 28 meat samples were collected from local groceries in Higashi-Hiroshima city, Hiroshima, Japan and tested for carbapenemases-encoding genes. Interestingly, 17 bacterial isolates were recovered and only one isolate confirmed to harbor both blaNDM-1 and blaVIM-1. To the best of our knowledge, this is the first report of carbapenem-resistant Klebsiella pneumoniae isolated from food in Japan that produces NDM-1 and VIM-1 carbapenemases. The strain was resistant to various antibiotics and harbored blaNDM-1, blaSHV-71, blaCTX-M-15, blaTEM-1, qnrS-1, and aac (6')-1b as well as two class 1 integrons: One containing blaVIM-1 and the other aadB-aadA2 and belongs to Sequence Type (ST) 30. In addition, blaNDM-1 was carried on an untypeable self-transmissible plasmid >90 kb in size. Our results are of a great interest and urge the authorities to consider the food as a potential source for carbapenemases and to comprise it for carbapenemase-producing organism’s surveillance programs.
Dutch Armed Forces/Royal Dutch Navy, Netherlands
Time : 11:00 AM - 11:45 AM
Works internationally for several medical and biotech companies as scientific advisory board member and is also an active reserve-officer of the Royal Dutch Navy in his rank as Commander (OF4). For the Dutch Armed Forces he is CBRNe specialist with focus on (micro)biological and chemical threats and medical- and environmental functional specialist within the 1st CMI (Civil Military Interaction) Battalion of the Dutch Armed Forces. For Expertise France he is now managing an EU CBRN CoE public health project in West Africa. He is visiting professor at the University of Rome Tor Vergata giving lectures for the CBRN Master study.
In his civilian position he is at this moment developing with MT-Derm in Berlin (Germany) a novel interdermal vaccination technology as well as a new therapy for cutaneous leishmaniasis for which he has won a Canadian ‘Grand Challenge’ grant. With Hemanua in Dublin (Ireland) he has developed an innovative blood separation unit, which is also suitable to produce convalescent plasma for Ebola Virus Disease therapy.
He has finished both his studies in Medicine and in Biochemistry in The Netherlands with a doctorate and has extensive practical experience in cell biology, immuno-haematology, infectious diseases, biodefense and transfusion medicine. His natural business acumen and negotiation competence helps to initiate new successful businesses, often generated from unexpected combinations of technologies.
Public health systems are not always prepared for outbreaks of infectious diseases. Although in the past several public health institutes, like the French ‘Institut Pasteur’ and the Dutch ‘Tropeninstituut‘, were prominent surveyors of infectious diseases, the investments in worldwide public health have decreased. Now more attention is given to curative healthcare compared to preventive healthcare. The recent Ebola Virus Disease outbreak in West Africa initiated a new wave of interest to invest in Worldwide Public Health to prevent outbreaks of highly contagious diseases.
Zoonotic diseases are threatening as the population does not have natural nor artificial (from vaccination) immune response to new diseases like in the Ebola Virus Disease outbreak in 2014. The new strain of the Ebola Virus in West Africa was slightly less lethal, compared to other Ebola Virus strains, but the threat of spreading was far bigger as it had a longer incubation time.
Most public health systems are not trained well enough to mitigate highly infectious and deadly disease outbreaks. NGO’s helping to fight the outbreak are often better trained in curative treatments and have less experience with biological (bioweapon) threats for which the military are trained for. The UNMEER mission was unique in this. It was a setting in which military and civilian actors cooperate in fighting a biological threat. Protection is essential for health workers. Smart systems have to be developed to prevent further spreading of the disease, but it is not only the biosafety, which has to be considered, but also the biosecurity, as misuse of extremely dangerous strains of microorganisms cannot be excluded.
Several zoonotic infectious diseases, like anthrax, smallpox and hemorrhagic fevers are listed as potential bioweapons. Therefor both biosafety and biosecurity have to be implemented in all measures to fight outbreaks of highly infectious diseases
University of Life Sciences in Lublin, Poland
Keynote: The role of consortia of microorganisms with plant extracts in protection and nutrition of plants
Time : 11:45 AM - 12:30 PM
Ewa Solarska is a Professor of University of Life Sciences in Lublin, Head of Laboratory of Organic Food and has experience in research R&D projects. Her research interests include evaluation of the quality of organic products, with particular emphasis on the content of mycotoxins in cereals and their products, development of technologies in organic production of selected agricultural and horticultural crops with using plant extracts fermented with microorganisms as protection agent. She has achievements in the field of processing of organic food with extended shelf life.
Microorganisms play an important role in plant health protection. Some of them are known as producers of bioactive substances such as vitamins, hormones, enzymes, antioxidants and antibiotics that can directly or indirectly enhance the growth and protection of plants. The use of microorganisms in consortia allows their better survival in any environment compared to single-species preparations, due to their synergism and the creation of a biofilm that is more effective for consortia with more efficient metabolism and more resistant to oxidative stress, as well as for toxic factors. Beneficial microorganisms that increase growth and protect plants can be added to the soil to improve the health of plants. The reason for this phenomenon is the close dependence of plants on their microbiome. By gaining better control over these relationships, pest growth can be reduced and the ecosystem can be more stable. Also fermented plant extracts that enhance plant defense mechanisms or directly toxic effects are used to increase the effectiveness of microorganisms in plant protection. In the University of Life Sciences in Lublin in 2012-2017 good practices were tested in the cultivation of hops, berries and vegetables in the organic system using Microorganism Consortia (MC) with fermented plant extracts. The biopreparations were used to spraying of plants to reduce the development of diseases and pests. Protection of hops and berries with the use of MC with fermented plant extracts has proved to be very effective, also in the case of dangerous pests such as aphids and spider mites. There was a higher content of micronutrients in vegetables that were sprayed with MC with fermented plant extracts. The use of MC with fermented plant extracts for the protection and nutrition of plants is an effective way to reduce diseases and pests and brings the expected results in the process of improving plant and soil quality and increasing the health value of food. This activity also has a very positive impact on the environment.
Enviro Technology Limited, India
Keynote: Industrial waste water treatment
Time : 12:30 PM - 13:15 PM
Maulin P Shah is currently working as the Chief Scientist and Head, Industrial Waste Water Research Lab, Division of Applied and Environmental Microbiology Lab at Enviro Technology Ltd., Ankleshwar, Gujarat, India. He has received his PhD in Environmental Microbiology from Sardar Patel University, Vallabh Vidyanagar, Gujarat. He has served as an Assistant Professor at Godhra, Gujarat University. He is a Microbial Biotechnologist with diverse research interest.
This study shows the pros and cons of using the combination of various technologies for industrial waste water treatment plant. Rapid industrialization, intensive agriculture and other human activities cause soil degradation, pollution and lowers the productivity and sustainability of the crops that further increase the pressure on natural resources and contribute to their degradation. Environmental bio remediation is an effective management tool for managing the polluted environment and in restoring the contaminated soil. The use of microbial sources, coupled with advanced technology is one of the most promising and economic strategies for the removal of environmental pollutants. There is a strong scientific growth with both the in situ and ex situ ways of bio remediation, in part due increased use of natural damping as most of the natural attenuation is due to bio degradation. The degradation of pollutants by environmental bio remediation technology can be a lucrative and environmentally friendly alternative. This article provides an overview of the important environmental bioremediation technologies and their application in treating the industrial waste water.